Simple neutral mixtures, which have one constituent in common, may be combined together straight away. This is very important for triple staining, which can only be attained by mixing together two simple neutral mixtures, each consisting of two components. A chemical decomposition need not be feared. We thus get mixtures containing three and more colours. Theoretically there are two possibilities for such combinations:
1. Staining mixtures of 1 acid and 2 basic dyes,
| e.g. orange—amethyst—methyl green; |
| narcëin—pyronin—methyl green; |
| narcëin—pyronin—methylene blue. |
2. Staining mixtures of 2 acids and 1 base, in particular the mixture to be described later in detail of
| orange g.—acid fuchsin—methyl green. |
| Further narcëin—acid fuchsin—methyl green, |
and the corresponding combinations with methylene blue, and amethyst violet may be mentioned.
The importance of these neutral staining solutions lies in the fact that they pick out definite substances, which would not be demonstrated by the individual components, and which we therefore call neutrophil.
Elements which have an affinity for basic dyes, such as nuclear substances, stain in these neutral mixtures purely in the colour of the basic dye; acidophil elements
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